细胞培养与作物品种改良

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植物细胞培养技术用于作物品种改良有巨大潜势。本文着重叙述生产同一性植物或诱导变异性。可从许多外植体繁殖植株，包括叶、花药、茎尖分生组织，甚至游离单细胞和原生质体。另一方法可建成愈伤组织或液体细胞悬浮培养体。当与常规作物改良规划相结合，细胞培养技术适用于1．离体培养繁殖，2．分生组织培养以消除病毒，3．次生产物合成，4．花药培养产生单倍体，和5．通过细胞或分子遗传学产生新品种。

【参考文献】：

〔1〕Aitken-Christie，J．and J．A．Gleed．1984 Uses for micropropagation of juvenile Radiata pine in New Zealand．In：Proc．Intcrn．Symp．of Recent Advances in Forest Biotechnol．(J．Hanover，D．Karnosky and D．Keathley，eds．)Michigan Biotechn．Institute，Traverse City，Michigan，June 10-13，1984，pp．47-57．

〔2〕Bonga，J．M．and D．J．Durzan eds．1982 Tissue Culture in Forestry，Martinus，Nijhoff，Dr．Junk，Amsterdam．The Netherlands，420pp．

〔3〕Brink，R．A．1962 Phase change in higher plants and somatic cell heredity．Q．Rev．Biol．37∶1-22．

〔4〕Durzan，D．J．1984 Potential for genetic manipulation of forest tree：totipotency，somaclonal aberration and trueness to type．In：proc．Intenn．Symp．Recent Advances in Forestry Techn．(J．Hanover，D．Karnusky，and D．Keathley，eds．) Michigan Bioechnology Institute，Traverse City，Michigan，June 10-13，1984，pp．117-139．

〔5〕Durzan，D．J．1984 Explant source：Juvenile and adult phases．In：Application of Plant Tissue Culture Methods for Crop Improvement．(D．V．Evans，W．R．Sharp，P．V．Ammirato，and Y．Yamada，eds．)Macmillian Publishing，New Jersey．

〔6〕Durzan，D．J．1980 Progress and promise in forest genetics In：Proc．Symp．on Paper Science and Technology-The Cutting Edge，50th Anniversary，The Institute of Paper Chemistry，Appleton，Wisconsin，pp．31-60．

〔7〕Hardy，R．W．1983 The Outlook for agricultural research and technology．In：Agriculture in the Twenty-First Century，J．W．Rosenblem ed．Wiley，Interscience，New York，pp．97-103．

〔8〕Tomes，D．T．1984 An assessment of the impact of biotechnology on plant breeding．In：Intern．Assoc．Plant Tissue Culture Newsletters No．42，pp．2-9．

〔9〕Durzan，D．J．1984 Special Problems：Adult vs．juvenile explants．Handbook of Plant Cell Culture，Vol．2．(W．R．Sharp，D．A．Evans，P．V．Ammirato and Y．Yamada，eds．)pp．471-504．Macmillian，New York．

〔10〕Wilkins，C．P．，J．L．Cabreba，P．and J．H．Dodds 1985 Tissue cult ure propagation of trees．Outlook on Agriculture vol．14 pP．2-13．