离体胚培养

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Hannig(1904)开创了胚培养的开发和农业应用新领域。他用完全无菌技术培养成熟胚(萝卜属与辣根菜属)于无机盐加糖培养基上，取得可移栽幼苗。从此曾应用胚培养以开发胚发育需要的营养和物理条件；避免种子休眠、缩短育种周期；测验种子生活力；提供微克隆材料源；拯救不亲和杂交的未成熟杂种胚，这是当今应用最广的。现已有72属植物胚离体培养成功。并有已发表过10余篇综合评论。本文介绍胚培养技术和评述培养要求和其应用。

【参考文献】：

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